Journal: Brain pathology (Zurich, Switzerland)

Article Title: Fra-1 induces apoptosis and neuroinflammation by targeting S100A8 to modulate TLR4 pathways in spinal cord ischemia/reperfusion injury.

doi: 10.1111/bpa.13113

Figure Lengend Snippet: Figure 7C,D, compared with the sham group, the num- ber of TUNEL-positive cells in the ventral horn of the spinal cord increased significantly in the rS100A8 and I/R groups. The results of the I/R + rS100A8 + TAK- 242 group versus I/R groups showed that the number of TUNEL-positive cells was significantly decreased (Figure 7C,D). Besides, our results revealed that the pro- tein levels of p-ERK/ERK, p-NF-κB/NF-κB and IL-1β were significantly decreased in the I/R + rS100A8+ TAK-242 group compared with that in the I/R group rats (Figure 7E,F). These results indicated that S100A8 pro- moted apoptosis and inflammation by activating the ERK1/2 and NF-κB pathways downstream of TLR4, and TAK-242 inhibited apoptosis induced by S100A8. IL-1β increased the expression of activated caspase-3 and apoptosis within infarcted forebrain area [43]. Taken together, the results from these in vivo and in vitro ana- lyses led us to hypothesize that microglia was activated by S100A8 secreted from neurons, promoted the

Article Snippet: Recombinant rat S100A8 (rS100A8, E. coli derived, with purity >95%) was purchased from Med Chem Express (China, cat. no. HY-P71275).

Techniques: TUNEL Assay, Expressing, In Vivo, In Vitro

Journal: Aging (Albany NY)

Article Title: S100A8 and S100A9, both transcriptionally regulated by PU.1, promote epithelial-mesenchymal transformation (EMT) and invasive growth of dermal keratinocytes during scar formation post burn

doi: 10.18632/aging.203112

Figure Lengend Snippet: S100A8 and S100A9 were upregulated in post burn skin and thermal-stimulated keratinocytes. ( A ) Representative blots for S100A8 and S100A9 in burned and matched normal skin tissues from burn patients. Comparison of ( B ) S100A8 and ( C ) S100A9 in burned and matched normal skin tissues of 38 patients. ( D ) Expression of S100A8 and S100A9 and ( F ) EMT marker proteins in thermal-stimulated keratinocytes was detected with Western blotting at different time points. ( E ) Double immunofluorescence assay for cellular localization of S100A8 and S100A9 and ( G ) immunofluorescence assay for nuclear translocation of Snail protein in thermal-stimulated keratinocytes at different time points. *** P < 0.001.

Article Snippet: Human recombinant S100A8 and S100A9 proteins (rS100A8 and rS100A9) were purchased form Abnova Corporation (Chinese Taipei).

Techniques: Comparison, Expressing, Marker, Western Blot, Immunofluorescence, Translocation Assay

Journal: Aging (Albany NY)

Article Title: S100A8 and S100A9, both transcriptionally regulated by PU.1, promote epithelial-mesenchymal transformation (EMT) and invasive growth of dermal keratinocytes during scar formation post burn

doi: 10.18632/aging.203112

Figure Lengend Snippet: S100A8 and S100A9 promote cell proliferation, expression of EMT marker proteins, and cell invasion in primary keratinocytes. Human primary keratinocytes were treated with 1 μg of rS100A8, 1 μg of rS100A9, 0.5 μg of rS100A8 plus 0.5 μg of rS100A9, or 1 μg of rS100A8 plus 1 μg of rS100A9, heat-inactivated rS100A8/rS100A9 were applied as a negative control. After incubation for 48 h, ( A ) cell number, ( B ) expression levels of EMT marker proteins, ( C ) proportion of CD44+CD24- cells, and ( D ) cell invasion, were respectively detected with automatic cell counter, Western blotting, FACS, and Transwell migration assay. * P < 0.05 vs. control, # P < 0.05 vs 1 μg rS100A8, $ P < 0.05 vs. 0.5 μg rS100A8 + 0.5 μg rS100A9.

Article Snippet: Human recombinant S100A8 and S100A9 proteins (rS100A8 and rS100A9) were purchased form Abnova Corporation (Chinese Taipei).

Techniques: Expressing, Marker, Negative Control, Incubation, Western Blot, Transwell Migration Assay, Control

Journal: Aging (Albany NY)

Article Title: S100A8 and S100A9, both transcriptionally regulated by PU.1, promote epithelial-mesenchymal transformation (EMT) and invasive growth of dermal keratinocytes during scar formation post burn

doi: 10.18632/aging.203112

Figure Lengend Snippet: PU.1 was positive associated with expression of S100A8/9 and identified as a transcription factor of them. ( A ) Transcriptionally activity of PU.1 on ( A ) S100A8 and ( B ) S100A9 gene promoters was detected by luciferase reporter gene assay. Pearson correlation coefficient assay was used to evaluate the correlations of ( C ) S100A8 and S100A9 expression levels, ( D ) S100A8 and PU.1 expression levels, and ( E ) S100A9 and PU.1 expression levels. ChIP-qPCR assay was used to validate the binding of PU.1 with ( F ) S100A8 promoter and ( G ) S100A9 promoter. ** P < 0.01.

Article Snippet: Human recombinant S100A8 and S100A9 proteins (rS100A8 and rS100A9) were purchased form Abnova Corporation (Chinese Taipei).

Techniques: Expressing, Activity Assay, Luciferase, Reporter Gene Assay, ChIP-qPCR, Binding Assay

Journal: Aging (Albany NY)

Article Title: S100A8 and S100A9, both transcriptionally regulated by PU.1, promote epithelial-mesenchymal transformation (EMT) and invasive growth of dermal keratinocytes during scar formation post burn

doi: 10.18632/aging.203112

Figure Lengend Snippet: S100A8 and S100A9 promoted scar development in PU.1 -/- burn model mice. ( A ) expression of PU.1, S100A8 and S100A9 proteins in the skin of WT and skin-specific PU.1 knockout (PU.1 -/- or ΔPU.1) mouse. The effect of overexpression of S100A8 or/and S100A9 on ( B ) expression of EMT marker proteins, ( C ) time of wound healing, ( D ) histopathological changes (by HE staining), ( E ) scar volume, ( F ) scar thickness, and ( G ) contents of collagens in PU.1 -/- burn model mice. * P < 0.05 vs. WT, # P < 0.05 vs. ΔPU.1+Vector.

Article Snippet: Human recombinant S100A8 and S100A9 proteins (rS100A8 and rS100A9) were purchased form Abnova Corporation (Chinese Taipei).

Techniques: Expressing, Knock-Out, Over Expression, Marker, Staining, Plasmid Preparation